cd-hit-est

Sequence file and databases

> Load Query Fasta file from your computer :

Sequence Identity Parameters
> Sequence identity cut-off :

Algorithm Parameters
> -r: comparing both strands

> -G: use global sequence identity

> -g: sequence is clustered to the best cluster that meet the threshold

> -b: bandwidth of alignment

Alignment Coverage Parameters

> -aL: minimal alignment coverage (fraction) for the longer sequence
> -AL: maximum unaligned part (amino acids/bases) for the longer sequence
> -aS: minimal alignment coverage (fraction) for the shorter sequence
> -AS: maximum unaligned part (amino acids/bases) for the shorter sequence
> -s: minimal length similarity (fraction)
> -S: maximum length difference in amino acids/bases(-S)
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Developed by @Zhipeng He