Welcome to HotSpot3D

Analyse Mutations in Protein 3D Structures

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ANALYSIS

Analysing Your Own Mutation Data for Clusters

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Coloum Number Name State Description
1 Hugo_Symbol Standard Hugo_symbols refer to the naming of human genes from the HUGO Gene Nomenclature Committee.
2 Chromosome Standard Chromosome field is a numeric value, for example, 18 indicates that the mutation appears on chromosome 18.
3 Start_Position Standard The starting position.
4 End_Position Standard The end position.
5 Variant_Classification Standard No need to screen for mutation types provided. Any variant classifications can for HotSpot3D, not just missense mutation.
6 Reference_Allele Standard Reference allele refers to the base that is found in the reference genome.
7 Tumor_Seq_Allele1 Standard
8 Tumor_Seq_Allele2 Standard
9 Tumor_Sample_Barcode Standard
10 transcript_name Non-standard A transcript ID column, referring to Ensembl coding transcript ID's (ENST).
11 amino_acid_change Non-standard A protein peptide change column (HGVS p. single letter abbreviations, ie p.T790M)

DATABASE

HotSpot3D, to identify spatial hotspots (clusters) and to interpret the potential function of variants within them.
We applied HotSpot3D to >4,400 TCGA tumors across 33 cancer types, and obtained the following results:
6,258

Clusters

800

Druggable Mutations

369

Rare Mutations

99

Medium Reccurence Mutations

HotSpot3D

HotSpot3D is a high-precision software for identifying high-frequency mutation hotspots in pan-cancer somatic cells.

Accessibility

HotSpot3D is a high-precision software for identifying high-frequency mutation hotspots in pan-cancer cells. HotSpot3D Web is dedicated to providing researchers with an easier way: you can quickly get software results using the HotSpot3D kernel with a single click of the prepared mutant data file.

Visualization and Re-visualization

HotSpot3D Web provides you with result data files and 3D visualization of the mutation hotspots. In addition, re-visualization is supported. Anytime, you can view mutation hotspots on protein 3D structure by uploading the results file.

Sharing

We applied HotSpot3D to TCGA to >4,400 TCGA tumors across 33 cancer types including BLCA, BRCA, CESC and so on. We directly provide results and visualization of clustering data on mutations here .

Contact Us

Beijing , China

chenshanyu@cnic.cn

+86 17610608763

Niu-LAB GitHub